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用双光子显微镜看看你的皮肤有没有重焕新生

标签: 显微镜皮肤
本帖最后由 汪洋玄远 于 2014-12-8 12:32 编辑

由材料人网编译团队华东分组李团伟翻译,陈召校正的《用双光子显微镜看看你的皮肤有没有重焕新生》的原文及翻译如下,亮点请点赞,槽点也记得拍砖哦!你的关注和评论,是我们前进的动力!
原文地址:http://www.materialsviews.com/measuring-skin-rejuvenescence/

In vivo and non-invasive: Collagen remodeling after micro-ablative fractional laser resurfacing can be monitored by two-photon microscopy.
无创在体内检测:双光子显微镜可以用于局部微蚀镭射磨皮后的胶原重塑的检测。

Various different cutaneous rejuvenation treatments, intended for use in anti-aging cosmetic surgery, have been developed in recent years, including a laser-based technique known as laser resurfacing. Skin irradiation with high-power pulsed laser light induces a thermal shock which stimulates fibroblasts to produce new collagen.This is arranged in a more ordered fashion with respect to the old, thus reducing wrinkles. To avoid long wound healing time and related risks, an improved method has been developed: micro-ablative fractional laser resurfacing ablates skin only on raster scanned points while the light energy dose deposited is still enough to activate fibroblasts and new collagen production.
近年来,已经发展了各种用于抗衰老整容手术的皮肤再生治疗方法,包括依托激光技术的镭射磨皮。其是利用高能脉冲激光照射皮肤,产生热震,刺激成纤维细胞产生新的胶原。其中规则排列的胶原与衰老相关,能够产生皱纹。为了避免较长伤口的愈合时间过长及相关的风险,现在发展了一种改进的方法:局部微蚀镭射磨皮法,其仅仅烧蚀光栅扫描点上的皮肤,而光所携带的能量仍能够刺激成纤维细胞产生新的胶原。

The macroscopic effect of such treatment can be measured by naked eye or a white light digital camera. At the microscopic level, it is very difficult to measure collagen modifications at a depth of 0.2 mm or deeper without a biopsy or a similarly invasive technique. Modern imaging techniques provide non-invasive alternatives.A team of Italian scientists from Florence, Fiorentino, and Rome used combined TPF-SHG microscopy to study the effects caused by micro-ablative fractional laser resurfacing.
这种治疗的宏观效果可以用肉眼或是白光数码相机检测。而在微观尺度水平上,如果没有组织切片或相似的创伤技术,我们是很难检测0.2mm或更深处胶原的改变。然而现代成像技术却给我们提供了一个非创伤性的选择。来自Florence, Fiorentino, and Rome 的意大利科学家团队使用组合的TPF-SHG显微镜,用它来研究局部微蚀镭射磨皮的效果。

Two-photon excited fluorescence (TPF) microscopy is a high-resolution laser scanning imaging technique enabling deep optical imaging of tissues.Additional morphological information can be provided by second-harmonic generation (SHG) microscopy, which can be combined with TPF microscopy using the same laser source.
双光子荧光(TPF)显微术是一种能够对深层组织进行光学成像的高分辨率激光扫描成像技术。另外, SHG显微镜可以与TPF显微镜结合使用同一个激光源。可由二次谐波发生(SHG)显微镜提供形貌信息。

For their study, the researchers performed in vivo two-photon imaging on the forearm of healthy subjects before and forty days after the laser treatment.De novo production of new collagen, as well as an increase in the amount of dermal amorphous component, was found within 40 days from the laser treatment. Visual inspection of the acquired SHG images of dermis by experienced dermatologists demonstrated a stronger collagen synthesis and remodeling on older subjects, whereas the modifications were minimal on younger subjects. The age-dependent effectiveness of the treatment was confirmed by a quantitative spectral analysis, based on the second-harmonic to autofluorescence aging index of dermis (SAAID), which is a recognized scoring method for skin aging assessment by means of non-linear microscopy. In addition, a pattern analysis of SHG images using grey-level co-occurrence matrix (GLCM) was carried out, a well-established method for scoring collagen organization in skin.
在他们的研究中,研究者在进行激光治疗前以及治疗后40天,对健康测试者的前臂进行活体双光子成像。(结果显示)激光处理后的40天里,新的胶原重新产生,真皮中无定型组分增加。由经验丰富的皮肤病专家肉眼观察获得的真皮SHG图像,证明了年长测试者的胶原更容易合成与重建,而年轻测试者的胶原的很难改变。根据真皮二次谐波自体荧光衰老指数(SAAID)及定量的光谱分析,可以确定的是治疗效果具有时间依赖性,而SAAID是一种公认的对由非线性显微镜方法进行皮肤老化评估的评分方法。另外,还使用了灰度共生矩阵(GLCM)的方法对SHG图像分析,GLCM是一种对皮肤胶原组织评分的通用方法。

While the diagnostic potential of in vivo multiphoton microscopy has already been demonstrated for skin cancer and other skin diseases, this study is the first to demonstrate its potential for a non-invasive follow-up of a laser-based treatment. It also represents a promising tool for the follow-up of other collagen-targeted therapies in dermatology. The researchers are convinced that in the near future non-linear laser scanning microscopy will find a stable place in the clinical dermatological setting.
与此同时,也证实了活体多光子显微镜对皮肤癌和别的皮肤病的诊断的可行性,该研究首次证明其作为基于激光无创治疗的后续诊断的可能性。它也有望成为别的胶原靶向皮肤病治疗的后续治疗的工具。研究者确信,不久以后非线性激光扫描显微镜将会应用到皮肤病的临床治疗上。

汪洋玄远

写了 201 篇文章,拥有财富 1553,被 31 人关注

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